
| COURSE
OF STUDY
M. Sc. in MOLECULAR & HUMAN GENETICS
(Semester System)
|
Candidates who have passed B.Sc. or B. Sc. (Hons) or B. Sc. (Ag.)
or M. B. B. S. from a recognized University or any other examination
considered equivalent will be eligible for admission to M. Sc. course
in Molecular & Human Genetics. The M. Sc. course shall be completed
in FOUR semesters.
|
| List
of Core, Major Elective and Minor Electives and their Credits
| CORE COURSES |
Theory |
Lab. Exercise |
MHG C 101
Transmission Genetics
|
3 (MHG
C 101 T) |
1 (MHG
C 101) L |
| MHG C 102
Basic Human Genetics |
3 (MHG
C 102 T) |
1 (MHG
C 102 L) |
| MHG C
103 Cell Biology |
2.5 (MHG
C 103 T) |
1 (MHG
C 103 L) |
| MHG C
104 Molecular Genetics I |
2.5 (MHG
C 104 T) |
0.5 (MHG
C 104L) |
| MHG C
105 DNA Technology & Genetic Engineering I |
2.5 (MHG
C 105 T) |
1 (MHG
C 105L) |
| MHG C 106
Biochemistry & Metabolic Disorders I |
2 (MHG
C 106 T) |
1 (MHG
C 106 L) |
| MHG C 107
Instrumentation |
1.5 (MHG
C 107 T) |
0.5 (MHG
C 107 L) |
| MHG C
208 Cytogenetics |
3 (MHG
C 208 T) |
1 (MHG
C 208 L) |
| MHG C 209
Molecular Genetics II |
2.5 (MHG
C 209 T) |
1 (MHG
C 209 L) |
| MHG C
210 DNA Technology & Genetic Engineering II |
2.5 (MHG
C 210 T) |
1 (MHG
C 210 L) |
| MHG C
211 Biochemistry & Metabolic Disorders II |
2 (MHG
C 211 T) |
1 (MHG
C 211 L) |
| MHG C 212
Model Genetic Systems |
1 (MHG
C 212 T) |
0.5 (MHG
C 212 L) |
| MHG C
213 Bioinformatics |
1 (MHG
C 213 T) |
1 (MHG
C 213 L) |
| MHG C
314 Human Molecular Genetics |
3 (MHG
C 314 T) |
1 (MHG
C 314 L) |
| MHG C
315 Developmental Genetics |
3 (MHG
C 315 T) |
1 (MHG
C 315 L) |
| MHG C 316
Behavioural Genetics |
1.5 (MHG
C 316 T) |
0.5 (MHG
C 316 L) |
| MHG C
317 Molecular Genetics of Human Pathogens |
1 (MHG
C 317 T) |
0.5 (MHG
C 317 L) |
| MHG C 418
Comprehensive Viva-voce |
|
2 (MHG
C 418 L) |
| TOTAL CREDITS
|
37.5
|
16.5
|
| MAJOR ELECTIVES
|
|
|
| MHG MA
301 Clinical Genetics |
3 (MHG
MA 301 T) |
1 (MHG
MA 301 L) |
| MHG MA
302 Immunogenetics |
2.5 (MHG
MA 302 T) |
0.5 (MHG
MA 302 L) |
| MHG MA
303 Population & Evolutionary Genetics |
2.5 (MHG
MA 303 T) |
|
| MHG MA
304 Genetic Counseling |
1 (MHG
MA 304 T) |
0.5 (MHG
MA 304 L) |
| MHG MA
405 Human Genome |
3 (MHG
MA 405 T) |
1 (MHG
MA 405 L) |
| MHG MA
406 Cancer Genetics |
1.5 (MHG
MA 406 T) |
0.5 (MHG
MA 406 L) |
| MHG MA
407 Clinical Assignments |
|
4 (MHG
MA 407 L) |
| MHG MA
408 Dissertation |
|
6 (MHG
MA 408 L) |
| TOTAL CREDITS
|
13.5
|
13.5
|
| MINOR ELECTIVES
|
|
|
| Drosophila
Genetics |
2 |
|
| MHG MI
02 Stress Biology |
2 |
|
| MHG MI
03 Reproductive Genetics |
2 |
|
| MHG MI
04 Seminar |
1.5 |
|
| MHG MI
05 Formulation of a Research Proposal |
1.5 |
|
| TOTAL
CREDITS |
9
|
|
(Note: The laboratory
exercises will be based on respective theory courses) |
|
M. Sc. in MOLECULAR & HUMAN GENETICS
Distribution of Courses in different Semesters
SEMESTER
I
| CORE (23) |
Theory |
Lab. Exercise |
| MHG C 101
Transmission Genetics |
3 |
1 |
| MHG C 102
Basic Human Genetics |
3 |
1 |
| MHG C 103
Cell Biology |
2.5 |
1 |
| MHG C 104
Molecular Genetics I |
2.5 |
0.5 |
| MHG C 105
DNA Technology & Genetic Engineering I |
2.5 |
1 |
| MHG C 106
Biochemistry & Metabolic Disorders I |
2 |
1 |
| MHG C 107
Instrumentation |
1.5 |
0.5 |
| Total |
17 |
6=23 |
SEMESTER
II
| CORE (17.5) |
Theory |
Lab. Exercise |
| MHG C 208
Cytogenetics |
3 |
1 |
| MHG C 209
Molecular Genetics II |
2.5 |
1 |
| MHG C 210
DNA Technology & Genetic Engineering II |
2.5 |
1 |
| MHG C 211
Biochemistry & Metabolic Disorders II |
2 |
1 |
| MHG C 212
Model Genetic Systems |
1 |
0.5 |
| MHG C 213
Bioinformatics |
1 |
1 |
| Minor Electives |
4 |
- |
| Total |
16 |
5.5=21.5 |
SEMESTER
III
| CORE (11.5) |
Theory |
Lab. Exercise |
| MHG C 314
Human Molecular Genetics |
3 |
1 |
| MHG C 315
Developmental Genetics |
3 |
1 |
| MHG C 316
Behavioural Genetics |
1.5 |
0.5 |
| MHG C 317
Molecular Genetics of Human Pathogens II |
1 |
0.5 |
| MAJOR ELECTIVES
(11) |
|
|
| MHG MA
301 Clinical Genetics |
3 |
1 |
| MHG MA
302 Immunogenetics |
2.5 |
0.5 |
| MHG MA
303 Population & Evolutionary Genetics |
2.5 |
- |
| MHG MA
304 Genetic Counseling |
1 |
0.5 |
| Total |
17.5 |
5=22.5 |
SEMESTER
IV
| CORE (2) |
Theory |
Lab. Exercise |
| MHG C 418
Comprehensive Viva-voce |
|
2 |
| MAJOR ELECTIVES
(16) |
|
|
| MHG MA
405 Human Genome |
3 |
1 |
| MHG MA
406 Cancer Genetics |
1.5 |
0.5 |
| MHG MA
407 Clinical Assignments |
- |
4 |
| MHG MA
408 Dissertation |
- |
6 |
| MINOR ELECTIVES |
5 |
- |
| Total |
9.5 |
13.5 =
23 |
| MINOR ELECTIVES
|
|
|
| MHG MI
01 Techniques in Drosophila Genetics |
2 |
|
| MHG MI
01 Techniques in Drosophila Genetics |
2 |
|
| MHG MI
02 Stress Biology |
2 |
|
| MHG MI
03 Reproductive Genetics |
2 |
|
| MHG MI
04 Seminar |
1.5 |
|
| MHG MI
05 Formulation of a Research Proposal |
1.5 |
|
|
CORE
COURSES
COURSE
MHG C 101: Transmission Genetics (3 + 1 Credits)
| 1 |
Introduction
to Genetics |
1 |
| 2 |
Mendelism
|
9 |
| |
2.1 Mendel
and his experiments
2.2 Law of segregation
2.3 Law of independent assortment
2.4 Application of laws of probability (product rule, sum rule)
2.5 Chromosomal basis of segregation and independent assortment
|
|
| 3 |
Chi-square
test and its application in analysis of genetic data |
1 |
| 4 |
Extensions
of Mendelism |
9 |
| |
4.1 Allelic
variation and gene function- Dominance relationships, basis
of dominant and recessive mutations
4.2 Multiple allelism, allelic series
4.3 Testing gene mutations for allelism: complementation test,
intragenic complementation
4.4 Visible, sterile and lethal mutations
4.5 Genotype to phenotype: effect of the environment on phenotype
development- Penetrance and expressivity, phenocopy
4.6 Gene interactions and modifying genes
4.7 Pleiotropy
|
|
| 5 |
Sex-linked
inheritance, Linkage and crossing over |
10 |
| |
5.1 Genetic
recombination and construction of genetic maps in Drosophila
5.2 Interference and coincidence
5.3 Cytological demonstration of crossing over in Drosophila
5.4 Mitotic recombination
5.5 Intragenic recombination
|
|
| 6 |
Inheritance
of quantitative traits |
6 |
| |
6.1 Continuous
and discontinuous variation
6.2 Polygenic inheritance
6.3 Genetic variance, heritability (narrow sense and broad sense)
|
|
| 7 |
Cytoplasmic
inheritance, maternal effects, inheritance due to parasites
and symbionts |
3 |
| Recommended
Books |
| 1 |
Atherly
et al
|
The
Science of Genetics
|
Saunders
|
1999 |
| 2 |
Brooker
|
Genetics –
Analysis and Principles
|
Benjamin/Cumings
|
1999 |
| 3 |
Fairbanks et al
|
Genetics
|
Wadsworth
|
1999 |
| 4 |
Gardner et al
|
Principles of Genetics
|
John Wiley
|
1991 |
| 5 |
Griffiths et al
|
Modern genetic Analysis
|
Freeman
|
2002 |
| 6 |
Griffiths et al
|
An Introduction
to Genetic Analysis
|
Freeman
|
2004 |
| 7 |
Hartl and Jones
|
Genetics –
Principles and Analysis
|
Jones & Bartlett
|
1998 |
| 8 |
Snustad et al
|
Principles of Genetics
|
Wiley and sons
|
1998 |
| 9 |
Strickberger
|
Genetics
|
Mcmillan
|
1985 |
| 10 |
Tamarin
|
Principles of Genetics
|
WCB
|
1996 |
COURSE
MHG C 102: Basic Human Genetics (3 + 1 Credits)
| 1 |
History
of Human Genetics |
1 |
| 2 |
Pedigrees- gathering
family history, pedigree symbols, construction of pedigrees,
presentation of molecular genetic data in pedigrees |
1 |
| 3 |
Monogenic traits |
12 |
| |
3.1 Autosomal inheritance-dominant,
recessive
3.2 Sex-linked inheritance
3.3 Sex-limited and sex-influenced traits
3.4 Mitochondrial inheritance
3.5 MIM number
3.6 Complications to the basic pedigree patterns- nonpenetrance,
variable expressivity, pleiotropy, late onset, dominance problems,
anticipation, genetic heterogeneity, genomic imprinting and
uniparental disomy, spontaneous mutations, mosaicism and chimerism,
male lethality, X-inactivation
3.7 Risk assessment; application of Bayes’ theorem
3.8 Allele frequency in population
3.9 Consanguinity and its effects |
|
| 4 |
Complex traits |
10 |
| |
4.1 Approaches to
analysis of complex traits- ‘Nature vs nurture’,
role of family and shared environment, monozygotic and dizygotic
twins and adoption studies
4.2 Polygenic inheritance of continuous (quantitative) traits,
normal growth charts, Dysmorphology
4.3 Polygenic inheritance of discontinuous (dichotomous) traits-
threshold model, liability and recurrence risk
4.4 Genetic susceptibility in multifactorial disorders (alcoholism,
diabetes mellitus, obesity)
4.5 Estimation of genetic components of multifactorial traits:
empiric risk, heritability, coefficient of relationship
|
|
| 5 |
Human cytogenetics |
10 |
| |
5.1 Techniques in
human chromosome analysis (including molecular techniques)
5.2 Human karyotype: banding, nomenclature of banding
5.3 Pathology of human chromosomes
5.4 Nomenclature of aberrant karyotypes
5.5 Common syndromes due to numerical chromosome changes
5.6 Common syndromes due to structural alterations (translocations,
duplications, deletions, microdeletion, fragile sites)
5.7 Common chromosome abnormalities in cancer
5.8 Genetics of fetal wastage |
|
| 6 |
Pharmacogenetics
and ecogenetics, teratogenetics |
5 |
| Recommended
Books |
| 1 |
Connor
& Smith
|
Essentials
of Medical Genetics
|
Blackwell
|
1993 |
| 2 |
Davies
|
Human Genetic Disease
Analysis
|
IRL
|
1993 |
| 3 |
Emery and Mueller
|
Elements of Medical
Genetics
|
ELBS
|
1992 |
| 4 |
Gersen & Keagle
|
The Principles of
Clinical Cytogenetics
|
Humana
|
1999 |
| 5 |
Jorde et al
|
Medical Genetics
|
Elsevier
|
1998 |
| 6 |
Korf
|
Human Genetics
|
Blackwell
|
1996 |
| 7 |
Lewis
|
Human Genetics
|
WCB
|
1999 |
| 8 |
Mange and Mange
|
Basic Human Genetics
|
Sinauer Assoc
|
1999 |
| 9 |
Maroni
|
Molecular and Genetic
Analysis of Human Traits
|
Blackwell |
2001 |
| 10 |
Nussbaum et al
|
Genetics in Medicine
|
Saunders
|
2001 |
| 11 |
Pasternak
|
An Introduction
to Molecular Human Genetics
|
Fritzgarald
|
2000 |
| 12 |
Prichard & Korf
|
Medical Genetics
at a glance
|
Blackwell
|
2004 |
| 13 |
Strachan and Read
|
Human Molecular
Genetics
|
Wiley
|
1999 |
| 14 |
Vogel and Motulsky
|
Human Genetics
|
Springer |
1997 |
COURSE
MHG C 103: Cell Biology (2.5 + 1 Credits)
| 1 |
Plasma
Membrane: Fluid mosaic model, structure of membrane, transport
across membrane and mechanisms of endocytosis and exocytosis |
4 |
| 2 |
Endomembrane system |
5 |
| |
2.1. General organization
of protein transport within and outside the cell
2.2. Protein sorting and secretion
2.3. Mechanism of intracellular digestion |
|
| 3 |
Cytoskeleton |
4 |
| |
3.1. Microfilaments:
Structural organization, cell motility and cell shape
3.2. Microtubule: Structural and functional organization, cilia,
flagella, centriole
3.3. Intermediate filaments |
|
| 4 |
Mitochondria |
10 |
| |
4.1 Ultrastructure
4.2 Chemiosmotic theory and respiratory chain complexes |
|
| 5 |
Structure and function
of peroxisome |
1 |
| 6 |
Structure and biosynthesis
of ribosome |
2 |
| 7 |
Cell cycle and its
regulation |
2 |
| 8 |
Cell-Cell Interaction
|
5 |
| |
8.1. Cell adhesion
molecules
8.2. Cellular junctions
8.3. Extracellular matrix |
|
| 9 |
Signal transduction |
5 |
| |
9.1. Intracellular
receptor and cell surface receptors
9.2. Signalling via G-protein linked receptors (PKA, PKC, CaM
kinase)
9.3. Enzyme linked receptor signaling (Growth factor receptor
signaling; JACK-STAT pathway)
9.4. Network and cross-talk between different signal mechanisms
|
|
| 10 |
Programmed cell
death |
1 |
| Recommended
Books |
| 1 |
Alberts
et al
|
Essential
Cell Biology
|
Garland
|
1998 |
| 2 |
Alberts et al |
Molecular Biology
of the Cell |
Garland
|
2002 |
| 3 |
Cooper
|
The Cell: A molecular
Approach
|
ASM Press
|
2000 |
| 4 |
Gilbert
|
Developmental Biology
|
Saunders
|
2003 |
| 5 |
Jeremy et al
|
Biochemistry
|
Freeman
|
2002 |
| 6 |
Karp
|
Cell and Molecular
Biology
|
John Wiley
|
2002 |
| 7 |
Lewin
|
Genes VIII
|
Pearson
|
2004 |
| 8 |
Lodish et al
|
Molecular Cell Biology
|
Freeman
|
2004 |
| 9 |
Pollard &Earnshaw |
Cell Biology
|
Saunders
|
2002 |
| 10 |
Tobin and Morcel
|
Asking about Cells
|
Saunders
|
1997 |
| 11 |
Wilson & Hunt
|
The Cell: A Problems
Approach
|
Garland
|
2002 |
COURSE
MHG C 104: Molecular Genetics I (2.5 + 0.5 Credits)
| 1 |
Properties
and evolution of genetic material, flow of genetic information |
2 |
| 2 |
Organization
of viral and bacterial genomes |
|
| 3 |
Eukaryotic
genome |
5 |
| |
3.1
C-value paradox
3.2 Repetitive DNA
3.3 General concept of a gene
3.4 Gene families
3.5 Non-coding genes |
|
| 4 |
Replication
in bacterial and eukaryotic chromosomes |
5 |
| |
4.1
DNA polymerases
4.2 Replicons, origin and termination
4.3 Replisome
4.4 Genes controlling replication |
|
| 5 |
Transcription |
5 |
| |
5.1
Prokaryotic RNA polymerase, sigma factors, initiation and termination
5.2 Eukaryotic RNA polymerases and their promoters
5.3 Processing of transcripts |
|
| 6 |
Translation |
2 |
| |
6.1
General mechanism
6.2 Role of rRNA in translation |
|
| 7 |
Regulation
of gene expression |
11 |
| |
7.1.
Regulation of transcription initiation |
|
| |
|
7.1.1.
Operon and regulon
7.1.2. Positive and negative regulation
7.1.3. Enhancers and promoters
7.1.4. Transcription factors: types, DNA binding motifs |
|
| |
7.2.
Regulation by attenuation and anti-termination |
|
| |
7.3.
Post transcriptional regulation |
|
| |
|
7.3.1. Alternative
splicing
7.3.2. Transport and targeting of RNA
7.3.3. Post-transcriptional gene silencing |
|
| |
7.4.
Translational control and targeting of proteins
7.5. Mechanism of steroid hormone and stress induced gene expressions |
|
| Recommended
Books |
| 1 |
Alberts
et al
|
The
Science of Genetics
|
Saunders |
1999 |
| 2 |
Benjamin
|
Genetics: A Conceptual
Approach
|
Freeman
|
2003 |
| 3 |
Berg and Singer
|
Genes and Genome
|
|
1998 |
| 4 |
Black
|
Microbiology: Principles
and Explorations
|
Wiley
|
2002 |
| 5 |
Dale & Schartz
|
From genes to Genome
|
Wiley & Sons |
2003 |
| 6 |
Griffiths et al
|
Modern Genetic Analysis
|
Freeman |
2002 |
| 7 |
Griffiths et al |
An Introduction
to Genetic Analysis
|
Freeman |
2004 |
| 8 |
Hartl et al
|
Essential Genetics
|
Wiley & Sons
|
2002 |
| 9 |
Klug & Cummings
|
Essentials of Genetics |
Prentice Hall
|
2003 |
| 10 |
Latchman
|
Gene Regulation
|
Chapman & Hall
|
1995 |
| 11 |
Lewin
|
Genes VIII
|
Pearson
|
2004 |
| 12 |
Maloy and Freifelder
|
Microbial Genetics
|
Jones and Barlett
|
1994 |
| 13 |
Meesfeld
|
Applied Molecular
Genetics
|
Wiley-Liss
|
1999 |
| 14 |
Ptashne
|
Genetic Switch
|
Blackwell
|
1986 |
| 15 |
Russell
|
Genetics
|
Benjamin
|
2002 |
| 16 |
Streips & Yasbin
|
Modern Microbial
Genetics
|
Wiley
|
2002 |
| 17 |
Trun & Trempy
|
Fundamentals of
Bacterial Genetics
|
Blackwell
|
2004 |
| 18 |
Watson et al
|
Molecular Biology
of the Gene
|
Pearson
|
2004 |
COURSE
MHG C 105: DNA Technology & Genetic Engineering I (2.5 + 1 Credits)
| 1 |
Enzymes
used in DNA technology |
4 |
| |
1.1 Restriction
and modification enzymes
1.2 Other nucleases
1.3 Polymerases
1.4 Ligase, kinases and phosphatases |
|
| 2 |
Nucleic acids |
4 |
| |
2.1 Isolation and
purification of DNA (genomic and plasmid) and RNA
2.2 Gel electrophoresis of nucleic acids (denaturing and native)
2.3 Pulse-field gel electrophoresis of DNA |
|
| 3 |
Cloning vectors |
7 |
| |
3.1 Plasmids
3.2 Phages
3.3 Cosmids
3.4 Artificial chromosomes
3.5 Shuttle vectors
3.6 Expression vectors |
|
| 4 |
Cloning techniques
|
5 |
| |
4.1 Construction
of genomic and cDNA libraries
4.2 Positional cloning: RFLP mapping, chromosome walking and
jumping |
|
| 5 |
Screening and characterization
of clones |
12 |
| |
5.1 Preparation
of probes
5.2 Restriction mapping
5.3 Principles of hybridizations and hybridization based techniques
(colony, plaque, Southern, Northern and in situ hybridizations)
5.4 Expression based screening
5.5 Interaction based screening: yeast two-hybrid system |
|
| Recommended
Books |
| 1 |
Ausubel
et al
|
Short
Protocols in Molecular Biology
|
Wiley
|
2002 |
| 2 |
Brown
|
Essential Molecular
Biology VI
|
AP
|
2000 |
| 3 |
Brown |
Essential Molecular
Biology VII
|
AP
|
2000 |
| 4 |
Brown |
Gene Cloning - An
Introduction |
Stanley Thornes |
1995 |
| 5 |
Glick and Pasternak |
Molecular Biotechnology |
ASM Press |
1998 |
| 6 |
Kracher |
Molecular Biology
- A Practical Approach |
|
|
| 7 |
Krenzer and Massey |
Recombinant DNA
and Biotechnology |
ASM |
2000 |
| 8 |
Micklos and Freyer |
DNA Science |
CSHL |
1990 |
| 9 |
Primrose |
Molecular Biotechnology |
Panima |
2001 |
| 10 |
Robertson et al |
Manipulation &
Expression of Recombinant DNA |
AP |
1997 |
| 11 |
Sambrook et al |
Molecular Cloning |
CSHL |
2001 |
| 12 |
Twyman |
Advanced Molecular
Biology |
Viva |
1999 |
| 13 |
Watson et al |
Recombinant DNA |
Freeman |
1992 |
COURSE
MHG C 106: Biochemistry & Metabolic Disorders I (2 + 1 Credits)
| 1 |
Amino
acids and peptides |
6 |
| |
1.1. Essential and
non-essential amino acids
1.2. Amino acids related disorders
1.3. Small peptides and their biomedical importance |
|
| 2 |
Proteins |
4 |
| |
2.1. Structure-conformation-function
relationship (exemplified by Myoglobin, Hemoglobin, and Collagen)
2.2. Protein degradation |
|
| 3 |
Enzymes |
8 |
| |
3.1. General properties
3.2. Kinetics: derivation of Michaelis-Menten equation, L-B
plot
3.3. Enzyme inhibition
3.4. Mechanism of action
3.5. Regulation of enzyme activity |
|
| 4 |
Carbohydrates |
8 |
| |
4.1. Mucopolysaccharides
and related disorders
4.2. Glycolysis
4.3. Krebs cycle
4.4. Gluconeogenesis
4.5. Glycogenesis and glycogenolysis,
4.6. Disorders of glycogen metabolism |
|
| Recommended
Books |
| 1 |
Berg
et al |
Biochemistry |
Freeman
|
2002 |
| 2 |
Bhagwan |
Medical Biochemistry |
AP |
2004 |
| 3 |
Devlin |
Biochemistry for
the Molecular Sciences |
Wiley |
2002 |
| 4 |
Higgins et al |
Biochemistry for
the Molecular Sciences |
Longman |
1994 |
| 5 |
Murray et al |
Harpers Illustrated
Biochemistry |
Prentice Hall |
2003 |
| 6 |
Nelson et al |
Lehninger’s
Principles of Biochemistry |
McMillan |
2000 |
| 7 |
Voet & Voet |
Biochemistry Vol.
1 and 2 |
Wiley
|
2004 |
| 8 |
Zubay et al |
Principles of Biochemistry |
WCB |
1995 |
COURSE
MHG C 107: Instrumentation (1.5 + 0.5 Credits)
| 1 |
Basic
knowledge of principles and applications of the following microscopy
techniques |
10 |
| |
1.1. Light Microscopy
1.2. Dark-field Microscopy
1.3. Phase-contrast Microscopy
1.4. Fluorescence Microscopy
1.5. Confocal Microscopy
1.6. Electron Microscopy
1.7. Photography, Digital imaging and image Processing |
|
| 2 |
Biochemical and
biophysical techniques |
10 |
| |
2.1. Centrifugation:
types of rotors, clinical, highspeed and ultracentrifuges
2.2. Colorimetry and spectrophotometry: Beer-Lambert law; absorption
spectrum, fluorescence spectrum, introduction to mass spectrometry
2.3. Chromatography: Paper, Thin layer chromatography; Columns:
ion-exchange, gel-filteration, HPLC and affinity columns, electrophoresis
2.4. Tracer techniques: Properties and units of radioactivity;
half-life; measurement of radioactivity by GM counter, liquid
scintillation counter; autoradiography Radio-immunoassay; safety
rules in handling of radioisotopes and hazardous chemicals |
|
| Recommended
Books |
| 1 |
Alberts
et al
|
Essential
Cell Biology
|
Garland
|
1998 |
| 2 |
Alberts et al
|
Molecular Biology
of The Cell
|
Garland |
2002 |
| 3 |
Boney
|
Cell Biology Level
II
|
Macdonald &
Evans
|
1982 |
| 4 |
Boyer
|
Modern Experimental
Biochemistry
|
Benjamin
|
1993 |
| 5 |
Clark & Switzer
|
Experimental Biochemistry
|
Freeman
|
2000 |
| 6 |
Freifelder
|
Physical Biochemistry
|
Freeman
|
1987 |
| 7 |
Karp
|
Cell and Molecular
Biology
|
John Wiley
|
2002 |
| 8 |
Locquin and Langeron
|
Handbook of Microscopy
|
Butterwaths
|
1983 |
| 9 |
Tobin and Morcel
|
Asking about Cells
|
Saunders
|
1997 |
COURSE
MHG C 208: Cytogenetics (3 + 1 Credits)
| 1 |
Chromatin
structure |
4 |
| |
1.1. Histones, DNA
1.2. Nucleosome and higher level organisation |
|
| 2 |
Chromosome organisation |
8 |
| |
2.1. Metaphase chromosome:
centromere and kinetochore, telomere and its maintenance; Holocentric
chromosomes and supernumerary chromosomes
2.2. Chromosomal domains (matrix, loop domains) and their functional
significance
2.3. Heterochromatin and euchromatin, position effect variegation,
boundary elements
2.4. Chromosome bandings |
|
| 3 |
Functional states
of chromatin and alterations in chromatin organization |
3 |
| 4 |
Structural and functional
organization of interphase nucleus |
2 |
| 5 |
Giant chromosomes |
4 |
| |
5.1 Polytene chromosomes
5.2 Lampbrush chromosomes |
|
| 6 |
Mitosis |
3 |
| |
6.1. Mitotic spindle
and arrangement of chromosomes on equator
6.2. Regulation of exit from metaphase
6.3. Chromosome movement at anaphase |
|
| 7 |
Meiosis |
5 |
| |
7.1 Overview of
the process
7.2 Meiosis specific cellular changes
7.3 Genetic control of meiosis (example: yeast) |
|
| 8 |
Chromosomal anomalies |
5 |
| |
8.1 Numerical
8.2 Structural
8.3 Meiosis in inversion and translocation heterozygotes; breakage-fusion-bridge
cycles
8.4 Induced chromosomal aberrations in somatic cells
8.5 Sister chromatid exchanges and somatic crossing over |
|
| 9 |
Dosage compensation
in Caenorhabditis, Drosophila and mammals |
5 |
| Recommended
Books |
| 1 |
Alberts
et al |
Essential
Cell Biology |
Garland |
1998 |
| 2 |
Alberts et al |
Molecular Biology
of The Cell |
Garland |
2002 |
| 3 |
Bostoc and Sumner |
The Eukaryotic Chromosome |
Elsevier |
1980 |
| 4 |
Hamsew and Flavell |
The Chromosome |
Bios |
1993 |
| 5 |
Hawley & Walker
|
Advanced Genetic
Analysis |
Blackwell |
2003 |
| 6 |
Hennig |
Structure &
Function of Eukaryotic Chromosomes
|
Springer |
1987 |
| 7 |
Lewin |
Genes VIII |
Pearson |
2004 |
| 8 |
Lodish et al |
Molecular Cell Biology |
Freeman |
| | |